Journal: Oncotarget
Article Title: miR-217 inhibits laryngeal cancer metastasis by repressing AEG-1 and PD-L1 expression.
doi: 10.18632/oncotarget.19121
Figure Lengend Snippet: Figure 7: Immunohistochemistry detection of clinical human laryngeal carcinoma samples. The number of AEG1 or PD- L1 positive cells was counted in 10 randomly chosen fields for each patient (magnification: ×200). Score: left 3+ and right 1+.
Article Snippet: Sections were then blocked with Protein Blocking Agent (Streptavidin-Biotin Universal Detection System, Beckman Coulter, Marseille, France) and incubated with the following primary antibodies overnight at 4 °C: rabbit anti-human AEG1 (13860-1- AP, 1:100, IgG, Proteintech group, USA) or rabbit antihuman PD-L1 (17952-1-AP, 1:100, IgG, Proteintech group, USA).
Techniques: Immunohistochemistry